Interaction Details


Details of experiments between Q9Y6Y8 and P56282
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SEC23IP
Fragment: 1-1000
Construct: endogenous proteins (Jurkat)

Protein B

Protein: POLE2
Fragment: 1-527
Construct: avi-his6-MBP-fusion protein
Average holdup BI: 0.33
Immobilized partner concentration (10-6M): 26
P value: 9.21
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-02-02
Number of measurements: 6
Measured pKd: 4.28
Experimental Details

Batch = Helsinki_batch6; Ncontrols = 12; Nsamples = 6; Npreys = 6515; threshold curvature: 2.18