Interaction Details


Details of experiments between Q9Y6N9 and Q92743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: USH1C
Fragment: 543-552
Site: PBM
Sequence: KEYDDELTFF
Construct: Biotin-Ahx-KEYDDELTFF

Protein B

Protein: HTRA1
Fragment: 365-480
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.25
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.3
PUBMED ID: 36115835