Interaction Details


Details of experiments between Q9Y6N9 and Q5T5U3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: USH1C
Fragment: 543-552
Site: PBM
Sequence: KEYDDELTFF
Construct: Biotin-Ahx-KEYDDELTFF

Protein B

Protein: ARHGAP21
Fragment: 47-159
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.87
PUBMED ID: 36115835