Interaction Details


Details of experiments between Q9Y6N9 and Q14CM0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: USH1C
Fragment: 543-552
Site: PBM
Sequence: KEYDDELTFF
Construct: Biotin-Ahx-KEYDDELTFF

Protein B

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.27
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.31
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.42
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: USH1C
Fragment: 208-299
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: USH1C
Fragment: 441-548
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: USH1C
Fragment: 75-178
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FRMPD4
Fragment: 1313-1322
Site: PBM
Sequence: RLPKIKETTV
Construct: biotin-ttds-RLPKIKETTV
Average holdup BI: 0
Immobilized partner concentration (10-6M): 6.6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835