Interaction Details


Details of experiments between Q9Y597 and Q14CM0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: KCTD3
Fragment: 806-815
Site: PBM
Sequence: SDSSGQEYSL
Construct: Biotin-Ahx-SDSSGQEYSL

Protein B

Protein: FRMPD4
Fragment: 67-160
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.39
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.45
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.69
PUBMED ID: 36115835