Interaction Details


Details of experiments between Q9Y566 and Q96BN8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SHANK1
Fragment: 655-761
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: OTULIN
Fragment: 343-352
Site: PBM
Sequence: PVRVCEETSL
Construct: Biotin-ado-ado-PVRVCEETSL
Average holdup BI: 0.78
Normalized holdup BI: 0.78
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.37
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: SHANK1
Fragment: 655-761
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: OTULIN
Fragment: 343-352
Site: PBM
Sequence: PVRVCEETSL
Construct: biotin-ttds-PVRVCEETSL
Average holdup BI: 0.68
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.68
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.14
PUBMED ID: 36115835