Interaction Details


Details of experiments between Q9Y4K4 and P00519
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAP4K5
Fragment: 384-398
Site: PxxP_1
Sequence: SKRAIPPPLPPKPRI
Construct: Bio Ado Ado-SKRAIPPPLPPKPRI-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.54
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: MAP4K5
Fragment: 1-846
Construct: endogenous protein (Jurkat)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration (10-6M): 10
P value: 0.03
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: MAP4K5
Fragment: 461-475
Site: PxxP_2
Sequence: EGSAQAPQLPRKKDK
Construct: Bio Ado Ado-EGSAQAPQLPRKKDK-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680