Interaction Details


Details of experiments between Q9Y4G8 and Q92743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RAPGEF2
Fragment: 1490-1499
Site: PBM
Sequence: EDEDEQVSAV
Construct: Biotin-Ahx-EDEDEQVSAV

Protein B

Protein: HTRA1
Fragment: 365-480
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Holdup BI standard deviation: 0
Normalized holdup BI: 0.2
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.18
PUBMED ID: 36115835