Interaction Details


Details of experiments between Q9Y4G8 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RAPGEF2
Fragment: 1490-1499
Site: PBM
Sequence: EDEDEQVSAV
Construct: Biotin-Ahx-EDEDEQVSAV

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Normalized holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.95
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: RAPGEF2
Fragment: 1490-1499
Site: PBM
Sequence: EDEDEQVSAV
Construct: Biotin-Ahx-EDEDEQVSAV

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.59
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: RAPGEF2
Fragment: 1490-1499
Site: PBM
Sequence: EDEDEQVSAV
Construct: Biotin-Ahx-EDEDEQVSAV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Holdup BI standard deviation: 0.02
Normalized holdup BI: -0.01
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835