Interaction Details


Details of experiments between Q9Y4G8 and P53671
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RAPGEF2
Fragment: 1490-1499
Site: PBM
Sequence: EDEDEQVSAV
Construct: Biotin-Ahx-EDEDEQVSAV

Protein B

Protein: LIMK2
Fragment: 143-248
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835