Interaction Details


Details of experiments between Q9Y3R0 and P59637
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRIP1
Fragment: 996-1086
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Average holdup BI: 0.17
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.04
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: GRIP1
Fragment: 145-239
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Average holdup BI: -0.07
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: GRIP1
Fragment: 242-344
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Average holdup BI: -0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: GRIP1
Fragment: 464-567
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Average holdup BI: 0
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: GRIP1
Fragment: 47-137
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Average holdup BI: -0.02
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: GRIP1
Fragment: 563-659
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Average holdup BI: -0.03
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: GRIP1
Fragment: 666-762
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov-E
Fragment: 67-76
Site: PBM
Sequence: SSEGVPDLLV
Construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Average holdup BI: 0
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835