Interaction Details


Details of experiments between Q9Y3R0 and P51812
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRIP1
Fragment: 464-567
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA3
Fragment: 731-740
Site: PBM
Sequence: GIKKITSTAL
Construct: biotin-ado-ado-GIKKITSTAL
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.62
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: GRIP1
Fragment: 996-1086
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA3
Fragment: 731-740
Site: PBM
Sequence: GIKKITSTAL
Construct: biotin-ado-ado-GIKKITSTAL
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.56
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: GRIP1
Fragment: 145-239
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA3
Fragment: 731-740
Site: PBM
Sequence: GIKKITSTAL
Construct: biotin-ado-ado-GIKKITSTAL
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.5
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: GRIP1
Fragment: 242-344
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA3
Fragment: 731-740
Site: PBM
Sequence: GIKKITSTAL
Construct: biotin-ado-ado-GIKKITSTAL
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 5

Protein A

Protein: GRIP1
Fragment: 563-659
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: RPS6KA3
Fragment: 731-740
Site: PBM
Sequence: GIKKITSTAL
Construct: biotin-ado-ado-GIKKITSTAL
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.