Interaction Details


Details of experiments between Q9Y3R0 and P22460
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRIP1
Fragment: 242-344
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.66
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: GRIP1
Fragment: 145-239
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.65
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: GRIP1
Fragment: 145-239
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: GRIP1
Fragment: 242-344
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: GRIP1
Fragment: 464-567
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: GRIP1
Fragment: 464-567
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: GRIP1
Fragment: 47-137
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: GRIP1
Fragment: 563-659
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: GRIP1
Fragment: 563-659
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: GRIP1
Fragment: 666-762
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: GRIP1
Fragment: 996-1086
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: GRIP1
Fragment: 996-1086
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCNA5
Fragment: 604-613
Site: PBM
Sequence: CLDTSRETDL
Construct: biotin-ttds-CLDTSRETDL
Average holdup BI: 0
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835