Interaction Details


Details of experiments between Q9Y3M2 and P62258
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CBY1
Fragment: 15-24
Site: 14-3-3_pSer20
Modification: (SEP)20
Sequence: PRKSASLSNL
Construct: PRKSA(pSer)LSNL (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.77
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 143
P value: 7.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.37
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: CBY1
Fragment: 15-24
Site: 14-3-3_pSer20
Modification: (SEP)20
Sequence: PRKSASLSNL
Construct: PRKSA(pSer)LSNL (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.72
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 116
P value: 7.94
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.34
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: CBY1
Fragment: 21-30
Site: 14-3-3_pSer26
Modification: (SEP)26
Sequence: LSNLHSLDRS
Construct: LSNLH(pSer)LDRS (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: -0.06
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 143
P value: 1.06
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: CBY1
Fragment: 2-11
Site: 14-3-3_pThr7
Modification: (TPO)7
Sequence: PFFGNTFSPK
Construct: PFFGN(pThr)FSPK (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.32
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 143
P value: 4.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.53
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: CBY1
Fragment: 2-11
Site: 14-3-3_pThr7
Modification: (TPO)7
Sequence: PFFGNTFSPK
Construct: PFFGN(pThr)FSPK (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.23
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 116
P value: 1.56
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))