Interaction Details


Details of experiments between Q9Y3M2 and P31946
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: CBY1
Fragment: 15-24
Site: 14-3-3_pSer20
Modification: (SEP)20
Sequence: PRKSASLSNL
Construct: PRKSA(pSer)LSNL (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.84
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 102
P value: 7.65
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.72
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: CBY1
Fragment: 15-24
Site: 14-3-3_pSer20
Modification: (SEP)20
Sequence: PRKSASLSNL
Construct: PRKSA(pSer)LSNL (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.85
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 134
P value: 8.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.62
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 3

Protein A

Protein: CBY1
Fragment: 21-30
Site: 14-3-3_pSer26
Modification: (SEP)26
Sequence: LSNLHSLDRS
Construct: LSNLH(pSer)LDRS (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: -0.27
Holdup BI standard deviation: 0.54
Immobilized partner concentration (10-6M): 134
P value: 0.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: CBY1
Fragment: 21-30
Site: 14-3-3_pSer26
Modification: (SEP)26
Sequence: LSNLHSLDRS
Construct: LSNLH(pSer)LDRS (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: -0.06
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 102
P value: 0.67
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: CBY1
Fragment: 2-11
Site: 14-3-3_pThr7
Modification: (TPO)7
Sequence: PFFGNTFSPK
Construct: PFFGN(pThr)FSPK (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.45
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 102
P value: 5
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.9
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: CBY1
Fragment: 2-11
Site: 14-3-3_pThr7
Modification: (TPO)7
Sequence: PFFGNTFSPK
Construct: PFFGN(pThr)FSPK (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.49
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 134
P value: 3.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.85
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))