Interaction Details

Details of experiments between Q9Y2U9 and Q04917
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: KLHDC2
Fragment: 205-214
Site: 14-3-3_pThr210
Modification: (TPO)210
Sequence: WSQPITTGKA
Construct: WSQPI(pThr)TGKA (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta

Measured dissociation constant (10^-6M):

Standard deviation of dissociation constant (10^-6M): 0

Average holdup BI: 0.2

Holdup BI standard deviation: 0.07

Normalized holdup BI: 0

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 130

P value: 3

Experiment method: HU Multiplex

Details of affinity fitting: hyperbolic binding equation

Date of measurement: 2021-10-13

Number of measurements: 5

The affinity was below the detection threshold of the assay.

DOI: 10.1101/2022.12.08.519103

Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))