Interaction Details


Details of experiments between Q9Y2H9 and Q9NR12
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: PDLIM7
Fragment: 448-457
Site: PBM
Sequence: LCKSHAFSHV
Construct: Biotin-Ahx-LCKSHAFSHV
Average holdup BI: 0.04
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.75
PUBMED ID: 36115835