Interaction Details


Details of experiments between Q9Y2H9 and Q8N743
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KIR3DL3
Fragment: 401-410
Site: PBM
Sequence: PKTPPTDTSV
Construct: biotin-ttds-PKTPPTDTSV
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.04
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KIR3DL3
Fragment: 401-410
Site: PBM
Sequence: PKTPPTDTSV
Construct: biotin-ttds-PKTPPTDTSV
Average holdup BI: -0.07
Normalized holdup BI: -0.13
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835