Interaction Details


Details of experiments between Q9Y2H9 and P06427
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.26
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.52
Normalized holdup BI standard deviation: 0.12
Immobilized partner concentration (10-6M): 20.2
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.78
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Sequence: SRSRRRETAL
Construct: biotin-ttds-SRSRRRETAL
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.48
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV33-E6
Fragment: 140-149
Site: PBM
Modification: (TPO)147
Sequence: SRSRRRETAL
Construct: Biotin-ttds-SRSRRREpTAL
Average holdup BI: -0
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835