Interaction Details


Details of experiments between Q9Y2H9 and O95477
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCA1
Fragment: 2252-2261
Site: PBM
Sequence: QDEKVKESYV
Construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.09
Normalized holdup BI: 0.2
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.16
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ABCA1
Fragment: 2252-2261
Site: PBM
Sequence: QDEKVKESYV
Construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.05
Normalized holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.79
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6