Interaction Details


Details of experiments between Q9Y2H9 and O75084
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Average holdup BI: 0.27
Normalized holdup BI: 0.53
Immobilized partner concentration (10-6M): 21.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.77
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAST1
Fragment: 965-1056
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FZD7
Fragment: 565-574
Site: PBM
Sequence: SHSSKGETAV
Construct: biotin-ttds-SHSSKGETAV
Average holdup BI: 0.13
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.45
PUBMED ID: 36115835