Interaction Details

Details of experiments between Q9Y2H0 and O14910
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: DLGAP4
Fragment: 983-992
Site: PBM
Sequence: IYVPEAQTRL
Construct: biotin-ttds-IYVPEAQTRL

Protein B

Protein: LIN7A
Fragment: 100-191
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.22

Normalized holdup BI: -0.22

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835