Interaction Details


Details of experiments between Q9Y2H0 and O14907
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DLGAP4
Fragment: 1-992
Construct: endogenous protein (Jurkat)

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: avi(biotin)-his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 10
P value: 0.22
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-08-03
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 36542723
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: DLGAP4
Fragment: 983-992
Site: PBM
Sequence: IYVPEAQTRL
Construct: biotin-ttds-IYVPEAQTRL

Protein B

Protein: TX1BP3
Fragment: 7-124
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.06
Holdup BI standard deviation: 0.12
Normalized holdup BI: -0.07
Normalized holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835