Interaction Details


Details of experiments between Q9UQL6 and Q04917
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HDAC5
Fragment: 254-263
Site: 14-3-3_pSer259
Modification: (SEP)259
Sequence: LRKTASEPNL
Construct: LRKTA(pSer)EPNL (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.93
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 130
P value: 8.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.99
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: HDAC5
Fragment: 542-551
Site: 14-3-3_pThr547
Modification: (TPO)547
Sequence: PRQPTTHPEE
Construct: PRQPT(pThr)HPEE (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.18
Holdup BI standard deviation: 0.1
Immobilized partner concentration (10-6M): 130
P value: 1.94
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))