Interaction Details


Details of experiments between Q9UPX8 and Q81964
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SHANK2
Fragment: 239-346
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.47
PUBMED ID: 36115835