Interaction Details


Details of experiments between Q9UPQ7 and Q7Z628
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: Biotin-ado-ado-SGGKRKETLV
Average holdup BI: 0.38
Normalized holdup BI: 0.45
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.7
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.4
Holdup BI standard deviation: 0.14
Normalized holdup BI: 0.43
Normalized holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.67
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
Measured pKd: 4.65
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Measured dissociation constant (10-6M): 31.58
Standard deviation of dissociation constant (10-6M): 2.57
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.5
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.2
Normalized holdup BI: 0.26
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.31
Experimental Details

not reported internal standards of holdup layout #5

Experiment series 6

Protein A

Protein: PDZRN3
Fragment: 405-515
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835