Interaction Details


Details of experiments between Q9UPQ7 and A0A1B0GV85
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PDZRN3
Fragment: 245-339
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: REELD1
Fragment: 517-526
Site: PBM
Sequence: PSVGSKKTVL
Construct: biotin-ado-ado-PSVGSKKTVL
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.48
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.