Interaction Details

Details of experiments between Q9ULJ8 and P0DTC4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PPP1R9A
Fragment: 499-593
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV

Average holdup BI: -0.01

Holdup BI standard deviation: 0.1

Immobilized partner concentration (10^-6M): 20

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835