Interaction Details


Details of experiments between Q9ULD6 and Q5VV41
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A

Protein: INTU
Fragment: 176-277
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ARHGEF16
Fragment: 700-709
Site: PBM
Sequence: MERLRVETDV
Construct: biotin-ttds-MERLRVETDV
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.32
PUBMED ID: 36115835