Interaction Details


Details of experiments between Q9ULB1 and Q9Y3R0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV

Protein B

Protein: GRIP1
Fragment: 996-1086
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Normalized holdup BI: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.09
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV

Protein B

Protein: GRIP1
Fragment: 145-239
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV

Protein B

Protein: GRIP1
Fragment: 242-344
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV

Protein B

Protein: GRIP1
Fragment: 464-567
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: NRXN1
Fragment: 1468-1477
Site: PBM
Sequence: KKNKDKEYYV
Construct: Biotin-Ahx-KKNKDKEYYV

Protein B

Protein: GRIP1
Fragment: 563-659
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835