Interaction Details


Details of experiments between Q9UKJ3 and P00519
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.68
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: GPATCH8
Fragment: 1-1502
Construct: endogenous protein (Jurkat)

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 10
P value: 2.96
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: ABL1
Fragment: 56-121
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680