Interaction Details


Details of experiments between Q9UKJ3 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.84
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.54
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: GPATCH8
Fragment: 1-1502
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.34
Immobilized partner concentration (10-6M): 10
P value: 6.51
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 4.72
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 3

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.48
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.76
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.8
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.48
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.78
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.38
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.71
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.52
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.05
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.84
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 5.55
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.75
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 5.3
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.71
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 5.38
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: GPATCH8
Fragment: 735-749
Site: PxxP_2
Sequence: PPGSGSPAPPRRRRR
Construct: Bio Ado Ado-PPGSGSPAPPRRRRR-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.53
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.86
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: GPATCH8
Fragment: 602-616
Site: PxxP_1
Sequence: HLQGLDPGEPNKSKE
Construct: Bio Ado Ado-HLQGLDPGEPNKSKE-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680