Interaction Details


Details of experiments between Q9UHC3 and Q6P0Q8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: Biotin-ado-ado-HRTCYLVTQL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.83
Normalized holdup BI: 0.83
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.53
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 4.25
Standard deviation of dissociation constant (10-6M): 0.1
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.37
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.84
Normalized holdup BI: 0.84
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.24
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.2
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.