Interaction Details


Details of experiments between Q9UHC3 and O75970
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1141-1246
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.35
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.73
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 371-467
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.04
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1624-1716
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.21
Normalized holdup BI: 0.21
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.91
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: Biotin-ado-ado-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1858-1949
Site: PDZ_12
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.89
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: Biotin-ado-ado-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1624-1716
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Normalized holdup BI: 0.1
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.8
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 688-793
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.4
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 7

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1858-1949
Site: PDZ_12
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 33
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.34
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 8

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 120-230
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 9

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1325-1441
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 10

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1464-1572
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 11

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1624-1716
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 12

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1722-1810
Site: PDZ_11
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 13

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1858-1949
Site: PDZ_12
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 14

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 1982-2070
Site: PDZ_13
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 15

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 246-342
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 16

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 548-634
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 17

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: Biotin-ado-ado-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 688-793
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.12
Normalized holdup BI: -0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 18

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 688-793
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 19

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: MPDZ
Fragment: 998-1105
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.