Interaction Details


Details of experiments between Q9UHC3 and O14745
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: NHERF1
Fragment: 10-98
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.6
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.23
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: ASIC3
Fragment: 522-531
Site: PBM
Sequence: HRTCYLVTQL
Construct: biotin-ttds-HRTCYLVTQL

Protein B

Protein: NHERF1
Fragment: 146-237
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Immobilized partner concentration (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.35
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.