Interaction Details


Details of experiments between Q9UGM3 and Q14155
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: ARHGEF7
Fragment: 178-251
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N