Interaction Details

Details of experiments between Q9UGM3 and P55345
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: -0.01

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: HU (DAPF)

Date of measurement: 2022-06-29

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 38995680

Experiment series 2