Interaction Details


Details of experiments between Q9UGM3 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DMBT1
Fragment: 1-2413
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.84
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 10
P value: 0.62
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.03
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.03
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 4

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.02
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.02
Holdup BI standard deviation: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Normalized holdup BI: -0.01
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 8

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.02
Holdup BI standard deviation: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 6
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.03
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 10

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.01
Holdup BI standard deviation: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.04
Holdup BI standard deviation: 0
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 12

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.02
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 6
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.05
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.05
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 15

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.01
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-08-04
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 17

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.02
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.01
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 9
P value: 0
Experiment method: HU (DAPF)
Details of affinity fitting: Bait concentration was adjusted to fit the profile to WT
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: DMBT1
Fragment: 215-229
Site: PxxP_1
Sequence: WPVRISPPVPTEGSE
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.02
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
P value: 0
Experiment method: HU (DAPF)
Date of measurement: 2022-10-24
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680