Interaction Details


Details of experiments between Q9Q6P4 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Protein B

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.4
Holdup BI standard deviation: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 4
Measured pKd: 4.61
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Protein B

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.29
Normalized holdup BI: 0.31
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.43
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Protein B

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.96
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Protein B

Protein: NHERF3
Fragment: 132-224
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Protein B

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: WNV-NS5
Fragment: 3424-3433
Site: PBM
Sequence: DTTLVEDTVL
Construct: biotin-(PEG)3-RYEDTTLVEDTVL

Protein B

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835