Interaction Details


Details of experiments between Q9P2F8 and P62258
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SIPA1L2
Fragment: 1467-1476
Site: 14-3-3_pSer1472
Modification: (SEP)1472
Sequence: GRRKFSFYGN
Construct: GRRKF(pSer)FYGN (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: -0.34
Holdup BI standard deviation: 0.24
Immobilized partner concentration (10-6M): 116
P value: 1.82
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: SIPA1L2
Fragment: 1467-1476
Site: 14-3-3_pSer1472
Modification: (SEP)1472
Sequence: GRRKFSFYGN
Construct: GRRKF(pSer)FYGN (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0
Holdup BI standard deviation: 0.13
Immobilized partner concentration (10-6M): 143
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: SIPA1L2
Fragment: 1483-1492
Site: 14-3-3_pSer1488
Modification: (SEP)1488
Sequence: LYRTLSDESI
Construct: LYRTL(pSer)DESI (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.06
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 116
P value: 0.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 4

Protein A

Protein: SIPA1L2
Fragment: 1483-1492
Site: 14-3-3_pSer1488
Modification: (SEP)1488
Sequence: LYRTLSDESI
Construct: LYRTL(pSer)DESI (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.06
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 143
P value: 1.03
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: SIPA1L2
Fragment: 157-166
Site: 14-3-3_pSer162
Modification: (SEP)162
Sequence: RQRSNSDVTI
Construct: RQRSN(pSer)DVTI (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.4
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 116
P value: 4.44
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.76
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: SIPA1L2
Fragment: 157-166
Site: 14-3-3_pSer162
Modification: (SEP)162
Sequence: RQRSNSDVTI
Construct: RQRSN(pSer)DVTI (crude synthetic peptide)

Protein B

Protein: YWHAE
Fragment: 1-255
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Average holdup BI: 0.35
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 143
P value: 4.14
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.57
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))