Interaction Details

Details of experiments between Q9P202 and Q9Y3R0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: DFNB31
Fragment: 813-904
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRIP1
Fragment: 1119-1128
Site: PBM
Sequence: LETREPTNTL
Construct: biotin-ado-ado-LETREPTNTL

Average holdup BI: 0.14

Normalized holdup BI: 0.14

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

Measured pK_d: 3.96

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: DFNB31
Fragment: 136-226
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRIP1
Fragment: 1119-1128
Site: PBM
Sequence: LETREPTNTL
Construct: biotin-ado-ado-LETREPTNTL

Average holdup BI: -0

Normalized holdup BI: -0

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: DFNB31
Fragment: 263-379
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: GRIP1
Fragment: 1119-1128
Site: PBM
Sequence: LETREPTNTL
Construct: biotin-ado-ado-LETREPTNTL

Average holdup BI: -0.01

Normalized holdup BI: -0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: DFNB31
Fragment: 565-578
Site: Internal_PBM
Construct: Biotin-GSGSGS-VSVDDVRSTSQGLSSY-CONH2

Protein B

Protein: GRIP1
Fragment: 145-239
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.05

Normalized holdup BI: -0.05

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: DFNB31
Fragment: 565-578
Site: Internal_PBM
Construct: Biotin-GSGSGS-VSVDDVRSTSQGLSSY-CONH2

Protein B

Protein: GRIP1
Fragment: 242-344
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.06

Normalized holdup BI: -0.06

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: DFNB31
Fragment: 565-578
Site: Internal_PBM
Construct: Biotin-GSGSGS-VSVDDVRSTSQGLSSY-CONH2

Protein B

Protein: GRIP1
Fragment: 464-567
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04

Normalized holdup BI: -0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: DFNB31
Fragment: 565-578
Site: Internal_PBM
Construct: Biotin-GSGSGS-VSVDDVRSTSQGLSSY-CONH2

Protein B

Protein: GRIP1
Fragment: 563-659
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.07

Normalized holdup BI: -0.07

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: DFNB31
Fragment: 565-578
Site: Internal_PBM
Construct: Biotin-GSGSGS-VSVDDVRSTSQGLSSY-CONH2

Protein B

Protein: GRIP1
Fragment: 996-1086
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.03

Normalized holdup BI: -0.03

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: DFNB31
Fragment: 898-907
Site: PBM
Sequence: FLVTEFNVML
Construct: biotin-ado-ado-FLVTEFNVML

Protein B

Protein: GRIP1
Fragment: 145-239
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.02

Normalized holdup BI: -0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 10

Protein A

Protein: DFNB31
Fragment: 898-907
Site: PBM
Sequence: FLVTEFNVML
Construct: biotin-ado-ado-FLVTEFNVML

Protein B

Protein: GRIP1
Fragment: 242-344
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04

Normalized holdup BI: -0.04

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 11

Protein A

Protein: DFNB31
Fragment: 898-907
Site: PBM
Sequence: FLVTEFNVML
Construct: biotin-ado-ado-FLVTEFNVML

Protein B

Protein: GRIP1
Fragment: 464-567
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.02

Normalized holdup BI: -0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 12

Protein A

Protein: DFNB31
Fragment: 898-907
Site: PBM
Sequence: FLVTEFNVML
Construct: biotin-ado-ado-FLVTEFNVML

Protein B

Protein: GRIP1
Fragment: 563-659
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.14

Normalized holdup BI: -0.14

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 13

Protein A

Protein: DFNB31
Fragment: 898-907
Site: PBM
Sequence: FLVTEFNVML
Construct: biotin-ado-ado-FLVTEFNVML

Protein B

Protein: GRIP1
Fragment: 996-1086
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.02

Normalized holdup BI: -0.02

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.