Interaction Details


Details of experiments between Q9P202 and Q9P0K1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DFNB31
Fragment: 136-226
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ADAM22
Fragment: 897-906
Site: PBM
Sequence: QSARLWETSI
Construct: biotin-ado-ado-QSARLWETSI
Average holdup BI: 0.79
Normalized holdup BI: 0.79
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.41
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: DFNB31
Fragment: 813-904
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ADAM22
Fragment: 897-906
Site: PBM
Sequence: QSARLWETSI
Construct: biotin-ado-ado-QSARLWETSI
Average holdup BI: 0.21
Normalized holdup BI: 0.21
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.2
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: DFNB31
Fragment: 263-379
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ADAM22
Fragment: 897-906
Site: PBM
Sequence: QSARLWETSI
Construct: biotin-ado-ado-QSARLWETSI
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.57
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.