Interaction Details


Details of experiments between Q9P202 and Q9BQQ3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DFNB31
Fragment: 898-907
Site: PBM
Sequence: FLVTEFNVML
Construct: biotin-ado-ado-FLVTEFNVML

Protein B

Protein: GORASP1
Fragment: 1-208
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.26
Normalized holdup BI: 0.26
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.32
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: DFNB31
Fragment: 565-578
Site: Internal_PBM
Construct: Biotin-GSGSGS-VSVDDVRSTSQGLSSY-CONH2

Protein B

Protein: GORASP1
Fragment: 1-208
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835