Interaction Details


Details of experiments between Q9NZW5 and Q9Y597
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MPP6
Fragment: 126-208
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: KCTD3
Fragment: 806-815
Site: PBM
Sequence: SDSSGQEYSL
Construct: Biotin-Ahx-SDSSGQEYSL
Average holdup BI: 0.11
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 36115835