Interaction Details


Details of experiments between Q9NZQ3 and P55345
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.67
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: NCKIPSD
Fragment: 1-722
Construct: endogenous protein (Jurkat)

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
P value: 0.04
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 4

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: PRMT2
Fragment: 24-96
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680