Interaction Details


Details of experiments between Q9NZQ3 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NCKIPSD
Fragment: 1-722
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.52
Immobilized partner concentration (10-6M): 10
P value: 4.99
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.03
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.43
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.67
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.26
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.68
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.51
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.82
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 22

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.23
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.52
PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 24

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 25

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.45
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.71
PUBMED ID: 38995680

Experiment series 27

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 29

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 30

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.49
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.77
PUBMED ID: 38995680

Experiment series 31

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 32

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 33

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 34

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.31
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.57
PUBMED ID: 38995680

Experiment series 35

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 36

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 37

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 38

Protein A

Protein: NCKIPSD
Fragment: 165-179
Site: PxxP_1
Sequence: LPSSQIPPQPRRAAP
Construct: Bio Ado Ado-LPSSQIPPQPRRAAP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 39

Protein A

Protein: NCKIPSD
Fragment: 173-187
Site: PxxP_2
Sequence: QPRRAAPTTPPPPVK
Construct: Bio Ado Ado-QPRRAAPTTPPPPVK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 40

Protein A

Protein: NCKIPSD
Fragment: 176-190
Site: PxxP_3
Sequence: RAAPTTPPPPVKRRD
Construct: Bio Ado Ado-RAAPTTPPPPVKRRD-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 41

Protein A

Protein: NCKIPSD
Fragment: 238-252
Site: PxxP_4
Sequence: SCSPTPPPVPRRGTH
Construct: Bio Ado Ado-SCSPTPPPVPRRGTH-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680