Interaction Details


Details of experiments between Q9NYP3 and Q92997
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DONSON
Fragment: 71-88
Construct: biotin-ttds-GPAAARRNPFARLDNRPR-CONH2

Protein B

Protein: DVL3
Fragment: 1-716
Construct: endogenous proteins (Jurkat)
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: -0.09
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 10
P value: 0.36
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic equation
Date of measurement: 2024-04-11
Number of measurements: 6
The affinity was below the detection threshold of the assay.
Experimental Details

Batch = Helsinki_batch7; Ncontrols = 24; Nsamples = 6; Npreys = 6872; threshold curvature: 4.55