Interaction Details

Details of experiments between Q9NYP3 and P18054
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: DONSON
Fragment: 71-88
Construct: biotin-ttds-GPAAARRNPFARLDNRPR-CONH2

Protein B

Protein: ALOX12
Fragment: 1-663
Construct: endogenous proteins (Jurkat)

Average holdup BI: -0.19

Immobilized partner concentration (10^-6M): 10

P value: 1.39

Experiment method: nHU_Jurkat

Details of affinity fitting: hyperbolic equation

Date of measurement: 2023-03-15

Number of measurements: 6

The affinity was below the detection threshold of the assay.

Experimental Details

Batch = Helsinki_batch2; Ncontrols = 18; Nsamples = 6; Npreys = 6313; threshold curvature: 1.21