Interaction Details


Details of experiments between Q9NYF3 and P31946
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FAM53C
Fragment: 227-236
Site: 14-3-3_pSer232
Modification: (SEP)232
Sequence: PQRRFSLSPS
Construct: PQRRF(pSer)LSPS (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.44
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 134
P value: 5.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.77
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: FAM53C
Fragment: 227-236
Site: 14-3-3_pSer232
Modification: (SEP)232
Sequence: PQRRFSLSPS
Construct: PQRRF(pSer)LSPS (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.38
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 102
P value: 5.59
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.77
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: FAM53C
Fragment: 249-258
Site: 14-3-3_pSer254
Modification: (SEP)254
Sequence: RSSPASSPEL
Construct: RSSPA(pSer)SPEL (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.74
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 102
P value: 9.91
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.44
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: FAM53C
Fragment: 249-258
Site: 14-3-3_pSer254
Modification: (SEP)254
Sequence: RSSPASSPEL
Construct: RSSPA(pSer)SPEL (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.72
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 134
P value: 6.8
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.29
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: FAM53C
Fragment: 294-303
Site: 14-3-3_pSer299
Modification: (SEP)299
Sequence: RRLRPSLDFD
Construct: RRLRP(pSer)LDFD (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.07
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 102
P value: 0.44
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))