Interaction Details


Details of experiments between Q9NYF3 and P61981
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: FAM53C
Fragment: 227-236
Site: 14-3-3_pSer232
Modification: (SEP)232
Sequence: PQRRFSLSPS
Construct: PQRRF(pSer)LSPS (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.5
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 6.75
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.87
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: FAM53C
Fragment: 227-236
Site: 14-3-3_pSer232
Modification: (SEP)232
Sequence: PQRRFSLSPS
Construct: PQRRF(pSer)LSPS (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.49
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 129
P value: 6.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.87
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: FAM53C
Fragment: 249-258
Site: 14-3-3_pSer254
Modification: (SEP)254
Sequence: RSSPASSPEL
Construct: RSSPA(pSer)SPEL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.83
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 129
P value: 10.44
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.58
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: FAM53C
Fragment: 249-258
Site: 14-3-3_pSer254
Modification: (SEP)254
Sequence: RSSPASSPEL
Construct: RSSPA(pSer)SPEL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.82
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 134
P value: 7.19
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.52
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: FAM53C
Fragment: 294-303
Site: 14-3-3_pSer299
Modification: (SEP)299
Sequence: RRLRPSLDFD
Construct: RRLRP(pSer)LDFD (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: -0.46
Holdup BI standard deviation: 0.78
Immobilized partner concentration (10-6M): 134
P value: 0.78
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: FAM53C
Fragment: 294-303
Site: 14-3-3_pSer299
Modification: (SEP)299
Sequence: RRLRPSLDFD
Construct: RRLRP(pSer)LDFD (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.17
Holdup BI standard deviation: 0.18
Immobilized partner concentration (10-6M): 129
P value: 0.98
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: FAM53C
Fragment: 227-236
Site: 14-3-3_pSer232
Modification: (SEP)232
Sequence: PQRRFSLSPS
Construct: PQRRF(pSer)LSPS (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.53
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 115
P value: 6.95
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: FAM53C
Fragment: 249-258
Site: 14-3-3_pSer254
Modification: (SEP)254
Sequence: RSSPASSPEL
Construct: RSSPA(pSer)SPEL (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.75
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 115
P value: 8.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.42
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 9

Protein A

Protein: FAM53C
Fragment: 294-303
Site: 14-3-3_pSer299
Modification: (SEP)299
Sequence: RRLRPSLDFD
Construct: RRLRP(pSer)LDFD (crude synthetic peptide)

Protein B

Protein: YWHAG
Fragment: 1-247
Site: 14-3-3
Modification: 100_uM_FCA
Construct: avi-his6-MBP-TEVsite-14-3-3gamma
Average holdup BI: 0.22
Holdup BI standard deviation: 0.21
Immobilized partner concentration (10-6M): 115
P value: 0.93
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))